Effects of probiotic supplementation on very low dose AFB1-induced neurotoxicity in adult male rats

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dc.authoridKARABULUT, DERYA/0000-0003-2067-6174
dc.contributor.authorSahin, Gizem Aytekin
dc.contributor.authorKarabulut, Derya
dc.contributor.authorUnal, Gokhan
dc.contributor.authorSayan, Meryem
dc.contributor.authorŞahin, Habibe
dc.date.accessioned2025-02-24T17:18:42Z
dc.date.available2025-02-24T17:18:42Z
dc.date.issued2022
dc.departmentFakülteler, Sağlık Bilimleri Fakültesi, Beslenme ve Diyetetik Bölümü
dc.description.abstractAims: Aflatoxin B1 (AFB1) is the most toxic and common form of AF found in food and feed. Although AFB1 exposure has toxic effects on many organs, studies on the brain are limited. Moreover, to the best of our knowledge, there is no study on the effect of probiotics on AFB1-induced neurotoxicity. Therefore, we aimed to evaluate the possible effects of probiotics on AFB1-induced neurotoxicity in the brain. Main methods: Thirty-two adult male Wistar rats were divided into four groups: Vehicle (VEH), Probiotic (PRO) (2.5 x 10(10) CFU/day VSL#3, orally), Aflatoxin B1 (AFB1) (25 mu g/kg/week AFB1, orally), and Aflatoxin B1 + Probiotic (AFB1 + PRO) (2.5 x 10(10) CFU/day VSL#3 + 25 mu g/kg/week AFB1, orally). At the end of eight weeks, rats were behaviorally evaluated by the open field test, novel object recognition test, and forced swim test. Then, oxidative stress and inflammatory markers in brain tissues were analyzed. Next, brain sections were processed for Hematoxylin & Eosin staining and NeuN and GFAP immunostaining. Key findings: Probiotic supplementation tended to decrease oxidative stress and inflammatory markers compared to the AFB1 group. Besides, brain tissues had more normal histological structures in VEH, PRO, and AFB1 + PRO groups than in the AFB1 group. Moreover, in probiotic groups, GFAP immunoreactivity intensity was decreased, while NeuN-positive cell number increased in brain tissues compared to the AFB1 group. Significance: Probiotics seem to be effective at reducing the neurotoxic effects of AFB1. Thus, our study suggested that especially Bifidobacterium and Lactobacillus species can improve AFB1-induced neurotoxicity with their antioxidant and anti-inflammatory effects.
dc.description.sponsorshipErciyes University Scientific Research Fund (ERU-BAP) [TDK-2019-9242]
dc.description.sponsorshipThis work was supported by the Erciyes University Scientific Research Fund (ERU-BAP, Project No: TDK-2019-9242) .
dc.identifier.doi10.1016/j.lfs.2022.120798
dc.identifier.issn0024-3205
dc.identifier.issn1879-0631
dc.identifier.pmid35843344
dc.identifier.scopus2-s2.0-85134499948
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.lfs.2022.120798
dc.identifier.urihttps://hdl.handle.net/20.500.14440/806
dc.identifier.volume306
dc.identifier.wosWOS:000834039600001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherPergamon-Elsevier Science Ltd
dc.relation.ispartofLife Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250201
dc.subjectAflatoxin B1
dc.subjectProbiotics
dc.subjectNeurotoxicity
dc.subjectBehavioral deficit
dc.subjectOxido-inflammation
dc.subjectHistopathology
dc.titleEffects of probiotic supplementation on very low dose AFB1-induced neurotoxicity in adult male rats
dc.typeArticle

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