Dentin bond strength and antimicrobial activities of universal adhesives containing silver nanoparticles synthesized with Rosa canina extract

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dc.authoridKaraaslan, Rabia/0009-0008-6823-1477
dc.contributor.authorEkrikaya, Semiha
dc.contributor.authorYilmaz, Ebubekir
dc.contributor.authorArslan, Soley
dc.contributor.authorKaraaslan, Rabia
dc.contributor.authorIldiz, Nilay
dc.contributor.authorCelik, Cagla
dc.contributor.authorOcsoy, Ismail
dc.date.accessioned2025-02-24T17:18:44Z
dc.date.available2025-02-24T17:18:44Z
dc.date.issued2023
dc.departmentFakülteler, Diş Hekimliği Fakültesi, Restoratif Diş Tedavisi Ana Bilim Dalı
dc.description.abstractObjective The purpose in the study was to evaluate the effect of biogenic silver nanoparticles (Ag NPs) synthesized by the green synthesis method on dentin bond strength in three different universal adhesives and investigate their antibiofilm activity against Streptococcus mutans (S. mutans).Materials and methods Three different universal adhesives (single bond universal, all-bond universal, and clearfil universal) were used in this study. Ag NPs were synthesized using rose hip (Rosa canina) extract as a reducing and stabilizing agent and they were characterized with STEM, UV-vis spectrophotometer, DLS, and zeta potential. Ag NPs were added to the adhesive resins at a rate of 0.05% (w/w), and their homogeneous distribution in the adhesive was determined using EDX spectrometry. Samples in all groups were tested at baseline-after 5000 and 10,000 thermal cycles. Adhesive composite discs were used for the live/dead analysis of S. mutans, MTT metabolic activity test, lactic acid production, and determination of colony-forming unit (CFU) values (n = 3). Ninety extracted caries-free human third molars were used to determine microtensile bond strength (mu TBS) (n = 10). After the universal adhesive was applied to the dentin surface, composite resin (Z550 XT, 3 M ESPE, USA) was placed and sections were taken to form a composite-dentin stick of 1 mm x 1 mm wideness and 8-mm length. The sticks were broken at a rate of 1 mm/min under uniaxial tension in a universal testing machine, and the failure modes were determined by SEM. One-way analysis of variance (ANOVA) for antibacterial tests and two-way analysis of variance for mu TBS tests were performed (p < 0.05).Results All universal adhesive groups containing Ag NPs showed higher antibacterial activity than control groups without Ag NPs (p < 0.05). There was a statistically significant difference in the live/dead assay analysis, MTT metabolic activity test, lactic acid production, and CFU values in the thermal cycled Ag NPs groups (p < 0.05). Antibacterial activity decreased in groups containing Ag NPs subjected to 10,000 thermal cycles. The highest lactic acid production 11.06 (+/- 0.629) and CFUs 7.61 (+/- 0.304), live bacteria 31.13 (+/- 0.466), and S. mutans MTT metabolic activity 0.29 (+/- 0.376) at AU (All-Bond Universal-Ag NPs) 10,000 thermal cycles group. There was no difference in mu TBS values between the initial and 5000 thermal cycle groups, there was a difference between the 10,000 thermal cycle groups. The CU (Clearfil Universal-Ag NPs) group subjected to 10,000 thermal cycles showed lower mu TBS 11.1 (+/- 3.2).Conclusion In conclusion, universal adhesives containing biogenic Ag NPs showed higher antibacterial activity than the control groups and did not reduce the mu TBS.
dc.description.sponsorshipTechnological and Scientific Council of Turkey (TUBITAK) [1139B411]
dc.description.sponsorshipThis study was funded by the Technological and Scientific Council of Turkey (TUBITAK) (project number:1139B411).
dc.identifier.doi10.1007/s00784-023-05306-6
dc.identifier.endpage6902
dc.identifier.issn1432-6981
dc.identifier.issn1436-3771
dc.identifier.issue11
dc.identifier.pmid37821654
dc.identifier.scopus2-s2.0-85173835772
dc.identifier.scopusqualityQ1
dc.identifier.startpage6891
dc.identifier.urihttps://doi.org/10.1007/s00784-023-05306-6
dc.identifier.urihttps://hdl.handle.net/20.500.14440/832
dc.identifier.volume27
dc.identifier.wosWOS:001084460500001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer Heidelberg
dc.relation.ispartofClinical Oral Investigations
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250201
dc.subjectAntibacterial activity
dc.subjectAg NPs
dc.subjectBond strength
dc.subjectUniversal adhesive
dc.subjectStreptococcus mutans
dc.titleDentin bond strength and antimicrobial activities of universal adhesives containing silver nanoparticles synthesized with Rosa canina extract
dc.typeArticle

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