The effects of melatonin and curcumin on the expression of SIRT2, Bcl-2 and Bax in the hippocampus of adult rats

Basit Öğe Kaydı
dc.authoridyazici mutlu, cigdem/0000-0002-6339-3054
dc.authoridKeskin-Aktan, Arzu/0000-0002-2878-0841
dc.authoridSONUGUR, FATMA GIZEM/0000-0002-2773-2872
dc.authoridOcal-Demirtas, Muge/0000-0002-9285-8244
dc.contributor.authorKeskin-Aktan, Arzu
dc.contributor.authorAkbulut, Kazime Gonca
dc.contributor.authorYazici-Mutlu, Cigdem
dc.contributor.authorSonugur, Gizem
dc.contributor.authorOcal, Muge
dc.contributor.authorAkbulut, Hakan
dc.date.accessioned2025-02-24T17:18:53Z
dc.date.available2025-02-24T17:18:53Z
dc.date.issued2018
dc.departmentFakülteler, Sağlık Bilimleri Fakültesi, Fizyoterapi ve Rehabilitasyon Bölümü
dc.description.abstractObjective: Though the mechanisms are not clearly understood, melatonin and curcumin have been reported to have neuroprotective effects. However, the mechanisms of neuroprotective effects of melatonin and curcumin in the brain are not clearly understood. In the current study, we investigated the effects of melatonin and curcumin treatments on oxidative stress parameters, the expression of SIRT2, Bcl-2 and Bax in the hippocampus. Methods: A total of thirty adult (13 months-old) male Wistar rats were divided into five groups: Control (1% ethanol:PBS), s.c. for 30 days), dimethyl sulfoxide (10%, s.c. for 30 days), Melatonin (10 mg/kg/day, s.c. for 30 days), Curcumin (30 mg/kg/day, i.p. for 30 days) and Salermide (100 mu M, i.p. for 30 days). The levels of malondialdehyde (MDA) glutathione (GSH) were measured as oxidative stress parameters in the hippocampus. The expression levels of SIRT2, Bcl-2 and Bax proteins were tested by western blotting and the SIRT2 protein levels of the hippocampal region was measured by a sandwich ELISA method. Results: Melatonin and curcumin significantly decreased MDA and SIRT2 expression in the hippocampus (p < 0.05). Accordingly, a significant increase in the GSH levels of curcumin-treated group and melatonintreated group was observed. Melatonin, but not curcumin, significantly increased the Bcl-2 expression of the hippocampal region. There was a significant correlation between SIRT2 and MDA levels (p < 0.05). Discussion: In conclusion, our results suggest that melatonin may increase cell survival in the hippocampus via decreasing oxidative stress and SIRT2 expression and increasing Bcl-2 expression.
dc.description.sponsorshipGazi University Scientific Research Project Foundation [01/2011-49]; Turkish Academy of Sciences (TUBA)
dc.description.sponsorshipGazi University Scientific Research Project Foundation (project no. 01/2011-49) and Turkish Academy of Sciences (TUBA) provided the financial support for this study.
dc.identifier.doi10.1016/j.brainresbull.2018.01.006
dc.identifier.endpage310
dc.identifier.issn0361-9230
dc.identifier.issn1873-2747
dc.identifier.pmid29325994
dc.identifier.scopus2-s2.0-85040329963
dc.identifier.scopusqualityQ2
dc.identifier.startpage306
dc.identifier.urihttps://doi.org/10.1016/j.brainresbull.2018.01.006
dc.identifier.urihttps://hdl.handle.net/20.500.14440/892
dc.identifier.volume137
dc.identifier.wosWOS:000427344300034
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherPergamon-Elsevier Science Ltd
dc.relation.ispartofBrain Research Bulletin
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250201
dc.subjectSIRT2
dc.subjectOxidative stress
dc.subjectMelatonin
dc.subjectCurcumin
dc.subjectHippocampus
dc.subjectSalermide
dc.titleThe effects of melatonin and curcumin on the expression of SIRT2, Bcl-2 and Bax in the hippocampus of adult rats
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Sağlık Bilimleri Fakültesi Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu