Molecular detection of E. faecalis in oral samples of a population associated with secondary endodontic infection

Basit Öğe Kaydı
dc.contributor.authorKesim, Bertan
dc.contributor.authorÜlger, Seda Tezcan
dc.contributor.authorCudal, Hamza
dc.contributor.authorAslan, Gönül
dc.contributor.authorErsoy, Leyla
dc.contributor.authorArslan, Tuğrul
dc.contributor.authorKüçük, Mustafa Öner
dc.date.accessioned2025-02-24T16:28:41Z
dc.date.available2025-02-24T16:28:41Z
dc.date.issued2021
dc.departmentFakülteler, Diş Hekimliği Fakültesi, Endodonti Ana Bilim Dalı
dc.description.abstractAim: The objective of this study was to evaluate the prevalence of\rEnterococcus faecalis in samples of oral rinse and tongue dorsum of\rendodontic patients with secondary/persistent infections (EPSI) using the\rPCR method.\rMethodology: Oral rinse samples (ORS) and tongue swab samples (TSS)\rof 22 patients (EPSI group) and 32 healthy individuals (control group) were\rcollected. DNA isolation from the TSS and ORS samples was performed\rusing the modified classical phenol-chloroform and chloroform method. To\rdetect E. faecalis strains directly from the TSS and ORS samples, the 310\rbase pair (bp) segment of the 16S rDNA of the E. faecalis genome was\ramplified by PCR using specific primers. The prevalence of E. faecalis was\rcompared between healthy and sick individuals using the Chi-square test,\rsignificance was set at p<0.05.\rResults: In the ORS samples, there was a significant difference between\rthe healthy individuals (n = 11, 34%) and the EPSI group (n = 15, 68%) in\rterms of the presence of E. faecalis (p = 0.026). In the TSS, the presence\rof E. faecalis was also investigated, and a significant difference was found\rbetween healthy individuals (n = 3, 9%) and the EPSI group (n = 11, 50%) (p\r= 0.001). In the EPSI group, no statistically significant difference was\rpresent in the prevalence rate of E. faecalis between the samples of ORS\r(68%) and TSS (50%) (p = 0.358).\rConclusion: The prevalence of E. faecalis was found to be statistically\rsignificantly higher in multi-site oral samples of a population with\rsecondary endodontic infection than healthy individuals.
dc.identifier.doi10.5577/intdentres.2021.vol11.no3.7
dc.identifier.endpage184
dc.identifier.issn2146-1767
dc.identifier.issue3
dc.identifier.startpage180
dc.identifier.trdizinid509261
dc.identifier.urihttps://doi.org/10.5577/intdentres.2021.vol11.no3.7
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/509261
dc.identifier.urihttps://hdl.handle.net/20.500.14440/334
dc.identifier.volume11
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofInternational Dental Research
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_TR-Dizin_20250201
dc.subjectDiş Hekimliği
dc.subjectMikrobiyoloji
dc.titleMolecular detection of E. faecalis in oral samples of a population associated with secondary endodontic infection
dc.typeArticle

Dosyalar

Koleksiyon

TR-Dizin İndeksli Yayınlar Koleksiyonu
Diş Hekimliği Fakültesi Koleksiyonu