Investigation of double-carbapenem efficiency in experimental sepsis of colistin-resistant Klebsiella pneumoniae

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dc.authoridEryilmaz-Eren, Esma/0000-0002-2712-9694
dc.contributor.authorKosar, Imran
dc.contributor.authorDinc, Gokcen
dc.contributor.authorEren, Esma
dc.contributor.authorAykemat, Yusuf
dc.contributor.authorKilic, Mesut
dc.contributor.authorKilic, Huseyin
dc.contributor.authorDoganay, Mehmet
dc.date.accessioned2025-02-24T17:19:09Z
dc.date.available2025-02-24T17:19:09Z
dc.date.issued2021
dc.departmentFakülteler, Sağlık Bilimleri Fakültesi, Beslenme ve Diyetetik Bölümü
dc.description.abstractOBJECTIVE: Klebsiella pneumoniae, a Gram-negative pathogen, especially which produces carbapenemase, is seen as a major threat to public health due to rapid plasmid-mediated spread of resistance and limited therapeutic options available for treatment. Although colistin has been recognized as a last resort antimicrobial for multidrug-resistant K. pneumoniae infections, these isolates have developed resistance to colistin as a result of its intensive use. The aim of this study was to evaluate the efficacy of double-carbapenem treatment of colistin-resistant K. pneumoniae experimental sepsis in mice. METHODS: In the study, 8-10-week-old Balb-c mice were divided as control groups (positive and negative) and treatment groups (colistin, ertapenem+meropenem, and ertapenem+meropenem+colistin). Sepsis was developed in mice by an intraperitoneal injection of colistin resistant K. pneumoniae. Antibiotics were given intraperitoneally 3 h after bacterial inoculation. Mice in each subgroup were sacrificed with overdose anesthetic at the end of 24-48 h and cultures were made from the heart, lung, liver, and spleen. Furthermore, homogenates of lung and liver were used to detect the number of colony-forming units per gram. Bacterial clearance was evaluated in lung and liver at different time points. RESULTS: When the quantitative bacterial loads in the lung and liver tissues are evaluated, no statistically significant difference was observed between different antibiotic treatments (p>0.05). All three treatment options were not effective, especially in 24 h. Only the decrease in bacterial load at the 48th h of the group treated with ertapenem + meropenem + colistin was found significant (p<0.05) compared to the 24 h. CONCLUSION: In the light of these data, it was understood that double-carbapenem application was not sufficient in the treatment of experimental sepsis in mice with colistin-resistant K. pneumoniae. Furthermore, ertapenem + meropenem + colistin combined therapy was not found to be superior to colistin monotherapy or double-carbapenem therapy.
dc.identifier.doi10.14744/nci.2020.14238
dc.identifier.endpage118
dc.identifier.issn2148-4902
dc.identifier.issue2
dc.identifier.pmid33851073
dc.identifier.scopusqualityQ4
dc.identifier.startpage113
dc.identifier.trdizinid410036
dc.identifier.urihttps://doi.org/10.14744/nci.2020.14238
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/410036
dc.identifier.urihttps://hdl.handle.net/20.500.14440/1028
dc.identifier.volume8
dc.identifier.wosWOS:000637256700001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakTR-Dizin
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherKare Publ
dc.relation.ispartofNorthern Clinics of Istanbul
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250201
dc.subjectCoastal resistance
dc.subjectexperimental sepsis
dc.subjectKlebsiella pneumoniae
dc.titleInvestigation of double-carbapenem efficiency in experimental sepsis of colistin-resistant Klebsiella pneumoniae
dc.typeArticle

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