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Öğe Biochemical effects of caffeine and melatonin on rat embryo brain(Journal of US-China Medical Science, 2020) Nisari, MustafaBackground:Oxidative stress plays an important role in fetus brain development. The aim of this study was to investigate the protective role of melatonin against the effect of caffeine (given 30 mg/kg and 60 mg/kg) in embryo brain.Material and Methods:For this purpose, 35 adult female Sprague-Dawley rats were used in the study. Pregnant rats were detected by vaginal smear test and were divided into seven groups with five rats per group.Thirty(30) mg/kg and 60 mg/kg caffeine were administered to the experimental groups between the 1st and 20th days of pregnancy, while melatonin (10 mg/kg) was administered to the treatment groups in addition to caffeine. On the 20th day of pregnancy, fetuses were taken by cesarean section and their weights/lengths were measured.Results:Superoxide dismutase (SOD), glutathione (GSH), glutathione disulfide (GSSG), total antioxidant status (TAS), thiobarbituric acid reactive substances (TBARS), calcium (Ca) and vitamin D (Vit D) levels were measured by spectrophotometric methods. Oxidative stress index (OSI) and total glutathione (GSH/GSSG) levels were the markers used for oxidative stress and measured by TOS/TAS and GSH/GSSG, respectively. All data were statistically evaluated.Conclusion:In this study, it was observed that high dose caffeine reduces oxidative stress in brain tissues while increasing the antioxidant level.Öğe CİVAN PERÇEMİ (Achillea millefolium)’nin DİYABETİK SIÇANLARIN KARACİĞER DOKUSUNDAKİ ANTİOKSİDAN ENZİMLER ÜZERİNE KORUYUCU ETKİSİNİN ARAŞTIRILMASI(2019) Nisari, MustafaAmaç: Civan perçemi (CP) antioksidan, antibakteriyel,diüretik ve anti-inflamatuar etkilere sahiptir. Çalışmamızda, streptozotosinle diyabet oluşturulan sıçan karaciğer dokularında MDA seviyesi ve antioksidan enzimaktivitesi üzerine CP’nin koruyucu etkisi araştırıldı.Gereç ve Yöntem: Çalışmada 250-300 gr 40 adet erkekWistar albino sıçanlar kullanıldı. Kontrol grubu, Diyabetgrubu ve CP ile tedavi edilen diyabet grubu olmak üzere3 gruba ayrıldı. Sıçanlarda diyabet oluşturmak için tekdoz 60 mg/kg streptozotosin (STZ) intraperitonealolarak enjeksiyon yapıldı. Tedavi grubuna ise 6 haftasüre ile 250 mg/kg/gün CP verildi. Deneyin sonundabütün hayvanlar sakrifiye edildi.Bulgular: Çalışma sonucunda streptozotosinle diyabetoluşturulmuş sıçanlarda kontrol grubu ile karşılaştırıldıklarında diyabet oluşturulan sıçanların karaciğerdokusundaki lipit peroksidasyon ürününde (MDA) veSOD enzim aktivitesinde, CAT ve GSH düzeylerindeistatistiksel olarak anlamlı derecede fark olduğu gözlendi (p<0.001.sırası ile). Bununla birlikte diyabetik sıçanlarda 6 hafta CP tedavisi sonrası, doku MDA seviyelerinde istatistiksel olarak anlamlı bir azalma gözlenirken,GST ve CAT enzim aktivitelerinde anlamlı bir artış(p<0.001.sırası ile) olduğu gözlendi.Sonuç: Diyabette oksidatif stresin indüklenmesininönemli olduğu düşünülmektedir. Bu çalışmada CP’ninantioksidan enzim aktivitelerinde artışa neden olduğundan diyabet durumunda lipid peroksidasyonunakarşı koruyucu etkilerinin olabileceğini göstermektedir.Öğe Civan percemi (Archilia millefolium)’nin diyabetik sıçanların karaciğer dokusundaki antioksidan enzimler üzerine koruyucu etkisinin araştırılması(Erciyes Üniversitesi, 2019) Nisari, MustafaAmaç: Civan perçemi (CP) antioksidan, antibakteriyel, diüretik ve anti-inflamatuar etkilere sahiptir. Çalışmamızda, streptozotosinle diyabet oluşturulan sıçan karaciğer dokularında MDA seviyesi ve antioksidan enzim aktivitesi üzerine CP’nin koruyucu etkisi araştırıldı. Gereç ve Yöntem: Çalışmada 250-300 gr 40 adet erkek Wistar albino sıçanlar kullanıldı. Kontrol grubu, Diyabet grubu ve CP ile tedavi edilen diyabet grubu olmak üzere 3 gruba ayrıldı. Sıçanlarda diyabet oluşturmak için tek doz 60 mg/kg streptozotosin (STZ) intraperitoneal olarak enjeksiyon yapıldı. Tedavi grubuna ise 6 hafta süre ile 250 mg/kg/gün CP verildi. Deneyin sonunda bütün hayvanlar sakrifiye edildi.Bulgular: Çalışma sonucunda streptozotosinle diyabet oluşturulmuş sıçanlarda kontrol grubu ile karşılaştırıldıklarında diyabet oluşturulan sıçanların karaciğer dokusundaki lipit peroksidasyon ürününde (MDA) ve SOD enzim aktivitesinde, CAT ve GSH düzeylerinde istatistiksel olarak anlamlı derecede fark olduğu gözlendi (p<0.001.sırası ile). Bununla birlikte diyabetik sıçanlarda 6 hafta CP tedavisi sonrası, doku MDA seviyelerinde istatistiksel olarak anlamlı bir azalma gözlenirken, GST ve CAT enzim aktivitelerinde anlamlı bir artış (p<0.001.sırası ile) olduğu gözlendi. Sonuç: Diyabette oksidatif stresin indüklenmesinin önemli olduğu düşünülmektedir. Bu çalışmada CP’nin antioksidan enzim aktivitelerinde artışa neden olduğundan diyabet durumunda lipid peroksidasyonuna karşı koruyucu etkilerinin olabileceğini göstermektedir.Öğe Does broccoli (brassica oleracea var. italica) extract have a potential therapeutic targets on human breast cancer (MDA-MB-231) using AgNOR detection method?(Sivas Cumhuriyet Üniversitesi, 2023) Çobanoğlu, Gözde; Nisari, Mustafa; Nisari, Mehtap; Uçar, Sümeyye; Kerek, Gülderen; İnanç, NerimanIn this study, it was aimed to determine the in vitro effect of the extract obtained from mature broccoli on the breast cancer cell line. MDA-MB231 breast cancer cell line was used in the study. MDA-MB-231 cells were exposed to broccoli extract at 37°C and 5% CO2 for varying durations (24 and 48 hours) and doses (125 and 250 µl/ml).At the end of the incubation period, viability, apoptosis, cell cycle and AgNOR protein status of MDA-MB-231 cells were examined in the Muse Cell Analyzer. In the groups containing broccoli extract, a decrease in the percentage of viable cells and a significant increase in the percentage of early and total apoptosis were observed for both doses compared to the control. In the cell cycle test, the number of cells in the S phase increased in all groups.It was observed that the groups containing broccoli extract slowed down the cell cycle in the transition to the S checkpoint. AgNOR staining results also supported cell cycle and apopitosis, and AgNOR number and TAA/NA ratio decreased in the 125 µl/ml broccoli extract group after 24-48 hours and were found to be statistically significant compared to the control group. It was determined that broccoli increased apoptosis on breast cancer cells by various mechanisms and inhibited cell viability/cell growth. The results were similar to the results of AgNOR protein synthesis. The study showed that the regular and correct consumption of broccoli could be effective in preventing cancer formation and slowing its progression.Öğe Does capsaicin have therapeutic benefits in human colon adenocarcinoma? Selection of the most reliable dose via AgNOR(Tubitak Scientific & Technological Research Council Turkey, 2020) Nisari, Mustafa; Eroz, RecepBackground/aim: To determine the effect of different doses of capsaicin on AgNOR protein synthesis in human colon adenocarcinoma derivate from colon cancer (Cato-2 cell). Materials and methods: In this experimental study, after the cultured of Caco-2 cell line, the cells are divided into 4 groups as control and different capsaicin exposed doses (25u mu, 50u mu, and 75u mu). Mean AgNOR number and total AgNOR area/nuclear area (TAA/NA) were calculated. Results: A significant differences were detected between control and capsaicin (50u mu) (P = 0.001), between control and capsaicin (75u mu) (P = 0.000), between capsaicin (25u mu) and capsaicin (50u mu) (P = 0.001) and between capsaicin (25u mu) and capsaicin (75u mu) (P = 0.000) for TAA/NA. Also, there were significant differences between control and capsaicin (50u mu) (P = 0.001), between control and capsaicin (75u mu) (P = 0.000), between capsaicin (25u mu) and capsaicin (50u mu) (P = 0.000) and between capsaicin (25u mu) and capsaicin (75u mu) (P = 0.000) for mean AgNOR number. Conclusion: A certain amount of capsaicin has a protective effect against colon adenocarcinoma and the dose concentrations are important for the most reliable treatment.Öğe Effect of Curcumin on Testis in Mice with Ehrlich Ascites Tumor(Fazile Nur Ekinci Akdemir, 2020) Yilmaz, Seher; Göçmen, Yeşim; Tokpınar, Adem; Ateş, Şükrü; Nisari, Mustafa; Caniklioğlu, Aysen; Öztekin, ÜnalIn the current work, the effects of curcumin on testicular tissues in Ehrlich Ascites Tumor (EAT) model developed in Balb/C mice. EAT cells (1x106) received from stock of animals were injected intraperitoneally to animals. 25mg/kg and 50mg/kg of curcumin was administered intraperitoneally. Testicular tissues obtained after all the experiment were evaluated for histopathological and biochemical parameters. Histopathological results showed that 50 mg/kg curcumin group had less EAT cells around testicular tissues than tumor control group. Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities and reduced glutathione (GSH), oxide glutathione (GSSG), total antioxidant status (TAS), total oxidant status (TOS), interleukin-1beta (IL-1?), interleukin-6, interleukin-17, tumor necrosis factor-alfa (TNF-?) levels were measured in testis tissues. Oxidative stress index (OSI) and GSH/GSSG ratio were calculated. Findings clearly suggested that antioxidant parameters (except CAT and GPx) had higher value in animal models treated with 25 and 50 mg curcumin groups associated to tumor control group. There was a statistically noteworthy variance between the groups in all parameters. Antitumor effect of curcumin on ascites tumor cells produced by EAT cells evidenced with histopathological while antioxidant and anti-inflammatory biochemical parameters evidenced with biochemical parameters.Öğe Effects of Different Doses of Propolis in Rats Which Were Formed Cold Stress on Anxiety to Investigate Sympathetic Skin Response and Elevated T Maze(Wiley, 2017) Nisari, Mustafa; Emre, Memet Hanifi; Dolu, Nazan; Acer, Hale; Pektas, FerhatÖğe Effects of Fenugreek Extract on Total Antioxidant/Oxidant Status at Ehrlich Ascites Tumor Bearing Mice(2020) Nisari, Mustafa; Alpa, Serife; Yilmaz, Seher; Inanç, NerimanObjectives: In this study, we aimed to effect of extract that derived from fenugreek on total antioxidant/oxidant statusat Ehrlich ascites tumor bearing Balb/C mice.Methods: The phenolic of Fenugreek extract was calculated as gallic acid equivalent using Folin-Ciocalteu method.Fenugreek extract concentration determined 200–400 mg/kg. In the end of in vivo studies, while the weight of experimental animals was estimated.Results: It was found that fenugreek extract delaying the weight gain. Administered fenugreek extract group hadlower serum TOS and OSI index and had higher serum TAS compared to the control group. These effects were higher inthe group receiving fenugreek 400 mg/kg intraperitoneally.Conclusion: As a result, the fenugreek extract shows antioxidant effect on EAT cells. We believe that our studies will beguiding for new studies about fenugreek and fenugreek could be advised as a food because of its antioxidant effect.Thus, it may have anticancer effect.Öğe Effects of home vacuum packaging method at different storage conditions on total antioxidant and phenolic compound levels in berry fruits(Walter De Gruyter Gmbh, 2025) Nisari, Mustafa; Başmısırlı, Eda; Aykemat, Yusuf; Aytekin-Sahin, Gizem; İnanç, Neriman; Dishan, AdaletThis study aimed to investigate the effects of home vacuum packaging on the total antioxidant capacity (TAC) and total phenolic content (TPC) levels of seven types of berries (raspberry, hawthorn, goldenberry, blackberry, strawberry, redcurrant, and blueberry) under different storage conditions. The berries were either vacuum+ or vacuum- and stored in a household refrigerator at +4 degrees C or frozen at -18 degrees C. Samples stored at +4 degrees C were analyzed on days 0, 7, and 30, while frozen samples were analyzed on days 0, 30, and 90. The results showed that TAC levels of fruit samples decreased by 18.88 % after 30 days at +4 degrees C in the vacuum- samples, while the vacuum+ samples showed only a 9.64 % reduction. Similarly, after 90 days of freezing, TAC levels decreased by 19.35 % in vacuum- samples, compared to an 11.56 % reduction in vacuum+ samples. Moreover, TPC levels of fruit samples decreased by 81.81 % after 30 days at +4 degrees C in the vacuum- samples, while the vacuum+ samples showed a 72.99 % reduction. After 90 days of freezing, TPC levels decreased by 75.99 % in vacuum- samples, while the vacuum+ samples showed only a 48.30 % reduction. Although TAC and TPC levels decreased over time at both +4 degrees C and -18 degrees C, the vacuum+ samples generally retained higher levels of TAC and TPC compared to the vacuum- samples, suggesting that vacuum packaging can slow down enzymatic and oxidative reactions. These findings indicate that home vacuum packaging could be a useful method for preserving the nutritional quality of berries and potentially reducing food waste.Öğe Effects of MTHFR C677T gene polymorphism on migraine together with biochemical and clinical parameters in turkish population(Mattioli 1885, 2020) Nisari, Mustafa; Baydemir, RecepTo detect the relation between methylenetetrahydrofolate reductase (MTHFR) gene C677T polymorphism and both of clinical and biochemical parameters. One hundred sixty migraine patients and one hundred twenty control group were included in the current study. Pain intensity of individuals were measured, biochemical, clinical parameters and MTHFR C677T single nucleotide polymorphism were detected. Statistically higher occurrence MTHFR C677T genotype was detected in migraine group than control group. A statistically significant association between family history of migraine and MTHFR C677T genotypes was detected. Also, statistically higher BMI, homocysteine and the total cholesterol levels were detected in MO and MA groups than control groups. When we take into consideration of the clinical parameters, only statistically significant difference was detected between MA and MO for attack frequency (attack/monthly). The triglyceride and homocysteine levels were significantly higher in males than females but HDL levels and folate were significantly higher in females than males. The frequency of CT genotype was significantly higher in cases with compression and allodynia than others in MA groups and cases with fatigue in both MA and MO subgroups. Also, cases without systemic complaints had significantly higher T allele frequency than cases with systemic complaints in the MA subgroup. Because the labor losses of patients with migraine may cause important economic losses, performed studies for the fully understanding of the disease including genetic and environmental factors are important for the prevention of negativity caused by the disease.Öğe Effects of Paclitaxel on Lipid Peroxidation and Antioxidant Enzymes in Tissues of Mice Bearing Ehrlich Solid Tumor(2019) Nisari, Mustafa; Kaymak, Emin; Ertekin, Tolga; Ceylan, Dilek; İnanç, Neriman; Özdamar, SaimObjectives: Cancer is the second most common cause of death in the world. Several chemotherapeutic drugs havebeen studied for their anticancer features. Paclitaxel is one of these chemotherapeutic drugs with high medicinal interest.This study was conducted in order to investigate effects of paclitaxel on lipid peroxidation and antioxidant enzymesin tissues of mice bearing solid-form Ehrlich tumors.Methods: In this study, 36 Balb/C male mice aged 8-10 weeks and weighing 25-30 g were used. Six mice were kept ascancer stock to produce Ehrlich Ascites Tumor (EAT) cells. Thirty mice were distributed into three groups as healthy control,tumor control and paclitaxel treatment. 0.1 ml physiologic saline solution was administered into mice in healthycontrol subcutaneously (s.c.) for 15 days. The animals in tumor control and Paclitaxel treatment groups received 1x106EAT cells s.c. through nape skin on the first day of the experiment. After the application of EAT cells, 10 mg/kg Paclitaxelwas injected intraperitoneally on days 4, 9 and 14. At the end of the study (on day 15), animals were sacrificed and liver,kidney, brain and testis tissues were excised and analyzed for Malondialdehyde (MDA), by using TBARS method, superoxidedismutase (SOD) and catalase (CAT) activities spectrophotometrically.Results: Compared to the results of healthy control group, tumor increased kidney and liver MDA levels developmentslightly but not significantly. Paclitaxel treatment significantly reduced the increased MDA levels in kidney and liver(p<0.001). Paclitaxel had no effect on testis MDA but brain MDA level reduced with the help of EAT cell injection andPaclitaxel returned the brain MDA level close to the level of healthy control (p<0.001). EAT cell injection reduced catalaseactivity in kidney and liver (p<0.001) and Paclitaxel had no effect on catalase activities in these tissues. In EAT cellinjected mice; testis and brain catalase activities were higher than that of healthy control group that were returned tocontrol levels by Paclitaxel treatment. Paclitaxel had no significant effect on decreased kidney and liver SOD activitieswhereas it significantly reduced the increased SOD activities in testis (p<0.05) and in brain (p<0.01).Conclusion: Paclitaxel alleviated the lipid peroxidation in kidney and liver but had no effect on antioxidant status inthese tissues of solid-Ehrlich tumor-bearing mice.Öğe İSKEMİ/REPERFÜZYON HASARI OLUŞTURULAN RATLARDA MELATONİNİN BÖBREK FONKSİYONLARI ÜZERİNE ETKİSİ(2018) Nisari, Mustafa; İnanç, Neriman; Soylu, Meltem; Yaşar, Yağmur; Başmısırlı, Eda; Kavutcu, MustafaAmaç: Serbest oksijen radikalleri iskemi/reperfüzyon(I/R) esnasında patofizyolojik doku değişimlerine neden olmaktadır. Bu çalışmada deneysel iskemi/reperfüzyonun sıçan böbreklerinde yol açtığı oksidatifhasara karşı güçlü bir antioksidan olan melatonininböbrek fonksiyonları ve böbrek hasarı üzerindeki etkilerinin araştırılması amaçlandı.Gereç ve Yöntem: Çalışmada 200-250 g ağırlığında 28adet dişi Wistar albino rat kullanıldı. Ratlar rastgele 4gruba ayrıldı (n=7). Kontrol grubu (K): Hayvanlara herhangi bir ilaç enjeksiyonu ya da I/R işlemi yapılmadı. I/R grubu: Herhangi bir ilaç enjeksiyonu yapılmadan solrenal arter 45 dakika iskemiye maruz bırakıldı veiskemiden çıkarılıp reperfüzyonundan emin olduktansonra kapatılarak, 24 saat yaşamalarına izin verildi.Melatonin grubu (Mel): Hayvanlara, 25 mg/kg dozundamelatonin i.p olarak enjekte edildi ve I/R işlemi uygulanmadı. I/R+Mel grubu: Gruptaki tüm hayvanlara 25mg/kg dozunda melatonin i.p olarak enjekte edilecek veenjeksiyondan 30 dakika sonra hayvanlar 45 dakikaiskemiye sokuldu, iskemiden sonra reperfüzyonunda 24saat yaşamasına izin verildi. Serumda Üre (BUN),Kreatinin (CRE) aktivitelerine, ürik asit, aspartataminotransferaz (AST) ve alanin aminotransferaz (ALT)seviyeleri değerlendirildi.Bulgular: I-R grubundaki üre, kreatinin, AST ve ALTseviyeleri K, M ve IR+M gruplarındaki seviyelerden daha yüksek olup istatistiksel olarak anlamlıydı (p<0.001). Bu durum böbreklerde İ/R hasarının oluştuğunugöstermektedir.Sonuç: Çalışma sonuçları iskemiye bağlı böbrek hasarının azaltılmasında Mel uygulamasının koruyucu etkisinin olabileceğini göstermektedir.Öğe MCF-7 ve MDA-MB-231 hücre hatlarında ginseng ekstresinin etkilerinin incelenmesi(2021) Nisari, Mustafa; Nisari , Mehtap; Gümüşsoy, Hacı Reşat; Uçar , Sümeyye; Nisarş, Mustafa[Abtsract Not Available]Öğe MDA-MB-231 Human Breast Cancer Cell Line Treated with Ginseng (Panax Quinquefolius): Evaluation by Annexin V and AgNOR Staining(2023) Gümüşsoy, Hacı Reşat; Nisari, Mustafa; Nisari, Mehtap; Uçar, Sümeyye; Koca, Fatih Mehmet; İnanç, NerimanAim: In this study, it was aimed to examine the time and dose dependent effects of Ginseng (Panax quinquefolius) on MDA-MB-231 cell lines. Material and Methods: MDA-MB-231 breast cancer cell line was used in the study. MDA-MB-231 cells were exposed to ginseng at 37°C and 5% CO2 for varying durations (24 and 48 hours) and doses (1 and 2 ?g/ml ginseng). At the end of the incubation period, viability, apoptosis, cell cycle and Argyrophilic nucleolar organizing region (AgNOR) protein status of MDA-MB-231 cells were examined in the Muse Cell Analyzer. Results: It was observed that the dose inducing apoptosis was 1 ?g/ml ginseng for 24 and 48 hours, and 2 ?g/ml ginseng for 48 hours in the group that stopped the cell cycle in the G0/G1 phase. When comparing the two groups; while no difference was determined between the control and 1 µg/ml ginseng groups, the significant differences were detected between the control and 2 µg/ml ginseng groups for mean AgNOR number in 48 hours incubation. However, there was no significant difference for the TAA/NA ratio, in the groups for 48 hours. Conclusion: The current study showed that ginseng had a crucial function against cancer development. Also, both AgNOR values might be used as biomarkers for detection of the most reliable therapeutic dose selection for cancer and it has been shown that correct consumption of Ginseng can be effective in preventing cancer formation and slowing its progression.Öğe Nuh Naci Yazgan Üniversitesi öğrencilerinin meyve ve sebze tüketim durumu ve ilişkin faktörler(2017) Başmısırlı, Eda; İnanç, Neriman; Nisari, Mustafa; Aykut, Mualla; Altunkürek, Şerife Zehra[Abtsract Not Available]Öğe Propolisin anksiyeteye ve bazı biyokimyasal parametrelere etkilerinin sempatik deri cevabı ve yükseltilmiş T labirent ile araştırılması(Fatih BAŞAK, 2020) Nisari, Mustafa; Emre, Memet; Dolu, Nazan; Acer, Hale; Pektaş, FerhatAmaç: Propolisin anti-mikrobiyal, antioksidan, anti-tümör, anksiyolitik ve anti-inflamatuar etkilere sahip olduğu gösterilmiştir. Ancak literatürde propolisin anksiyojenik etkisi konusundaki çalışmalara rastlanılmamıştır. Bu çalışmamızda, soğuk stresi oluşturulmuş sıçanlarda propolisin farklı dozlarının anksiyeteye etkilerinin sempatik deri cevabı (elektrodermal aktivite) ve yükseltilmiş T labirent ile araştırılması amaçlandı.Yöntemler: Çalışmada 40 adet Wistar albino erkek sıçan kullanıldı. Kontrol grubu, düşük doz (10 mg/kg PRO), orta doz (30 mg/kg PRO) ve yüksek doz propolis (50 mg/kg PRO) grupları oluşturularak, kontrol grubu hariç diğer gruplara propolis gavaj yoluyla uygulandı. Enjeksiyondan 20 dakika sonra, sıçanların anksiyete skorları yükseltilmiş T-labirent ile değerlendirildi ve daha sonra da elektrodermal aktiviteleri (EDA) ölçüldü. Deney sonunda kan örneklerinde, spektrofotometrik olarak malondialdehit (MDA) ile bazı enzimatik ve lipid değerleri ölçüldü.Bulgular: 10 mg/kg PRO grubunda açık kolda harcanan zamanın yüzdesi ve açık kola giriş sayısı, diğer gruplara göre daha düşük bulunurken 30 mg/kg PRO grubunda artış gözlendi. EDA, 30 mg/kg PRO (sırasıyla, P=0,012, P=0,02) ve 50 mg/kg PRO (sırasıyla, P=0,013, P=0,02 gruplarında kontrole kıyasla daha düşüktü. MDA’nın 30 mg/kg PRO ve 50 mg/kg PRO gruplarında önemli oranda düşük olduğu görüldü. 30 mg/kg PRO grubunda AST değeri artış gösterirken ALT değerinde azalmanın olduğu görüldü. Toplam kolesterol ve trigliserit değerleri 50 mg/kg PRO grubunda anlamlı olarak düşüktü. HDL değerinin düşük doz propolisten uygulamasından itibaren anlamlı yükseldiği, LDL değerinin ise sadece 10 mg/kg PRO grubunda anlamlı azaldığı olduğu görüldü.Sonuçlar: EDA ve T-labirent yöntemleriyle elde edilen sonuçlara göre düşük ve orta doz propolis anksiyetik etki yaparken, yüksek doz propolis anksiyolitik etki göstermiştir. Ayrıca sıçanlara soğuk stres sonucunda uygulanan propolisin MDA değerlerini orta ve yüksek doz gruplarında düşürdüğü, enzimatik ve lipid değerlerinde ise organizma lehine olumlu değişimlere sebep olduğu görüldü. Dolayısıyla propolisin anksiyeteye ilişkin yanıtlarının, doza bağlı etkilerine göre değiştiği sonucuna varılmıştır.Öğe Rhamnetin improves antioxidant status in the liver of Ehrlich solid tumor bearing mice(Medical Science and Discovery, 2020) İnanç, Neriman; Nisari, Mustafa; Güneş, Ömer; Soylu, Meltem; Tascılar, Emre; Serdar, Muhittin A.Objective:Rhamnetin, a flavanol, is in the subclasses of the flavonoids existing in plants. The antioxidant properties of several plants containing flavonoids have been extensively studied in several diseases including cancer. This study investigated the effects of rhamnetin on tumor masses, oxidant and antioxidant status in the livers of mice bearing Ehrlich solid tumor. Material and Methods:Fifty male Balb/C mice weighing 25-30 g were used in the study. Ten mice were keptfor Ehrlich ascites tumor (EAT) cells production and the remaining mice were randomly assigned to four groups containing 10 mice in each as healthy control and treatments receiving 1x106 EAT cells and EAT cells plus 100 ?g/kg/day or 200 ?g/kg/day rhamnetin via subcutaneous route. The tumor inhibition rates of rhamnetin treatments were calculated. The livers were analyzed for malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) levels. Results:Compare to tumor control, both levels of rhamnetin suppressed tumor masses throughout the experiment. The MDA levels were increased whereas SOD and CAT activities were reduced by EAT cells injection in the liver of mice. The 100 ?g/kg/day rhamnetin treatment decreased MDA level but 200 ?g/kg/day rhamnetin had no significant effect on increased MDA level. The reduced liver SOD (p<0.001) and CAT (p<0.01) activities were elevated by both levels of rhamnetin injection. Conclusions:The results of this study have revealed that rhamnetin suppresses tumor progression and improves antioxidant status in the livers of solid tumor-bearing miceÖğe Sağlık ve fen bilimlerine temel ve klinik yaklaşımlar(İKSAD, 2020) Nisari, Mustafa[Abtsract Not Available]Öğe THE EFFECT OF CAROB (CERATONIA SILIQUA L.) AGAINST NICOTINE BASED OXIDATIVE STRESS(2024) Nisari, Mustafa; Yılmaz, Seher; Göçmen, Ayşe Yeşim; Öztekin, Ünal; Ateş, Şükrü; Şeker Karatoprak, Gökçe; İnanç, NerimanOBJECTIVE: In this study the antioxidant effect of carob plant, ıt's effects of nicotine-induced oxidative stress and the number of pyramidal cells in the rat hippocampus in cornu ammonis (CA) were investigated. MATERIAL AND METHODS: In the study, 28 adult Wistar Albino male rats were used. Rats are divided into four groups as control group, carob group, nicotine group and nicotine + carob group. Nicotine was applied to the experimental group, and carob extract was applied to the treatment group as well as nicotine. Superoxide dismutase (SOD), glutathione (GSH), glutathione disulfide (GSSG), total oxidant capacity (TOS), total antioxidant capacity (TAS) values were measured by spectrophotometric analysis on the lung, brain, kidney, heart and liver tissues. Oxidative stress index (OSI) and GSH / GSSG values, respectively, were calculated as TOS/TAS and GSH/GSSG rates. The number of pyramidal cells in the CA was estimated using the optical fractionator technique. RESULTS: It is seen that the TAS level in the kidney tissue of the nicotine group is significantly lower than the control and carob groups. In brain tissue, the TAS level of the nicotine group was significantly lower than that of other groups (p<0.001). The OSI value of the nicotine group was significantly higher in liver tissue compared to the control group (p<0.001). Nicotine exposure has been shown to cause a significant reduction in the number of pyramidal cells in CA. CONCLUSIONS: It is understood that Carob plant is an important phytomedical plant that has antioxidant properties against nicotine by increasing TAS level in oxidative stress formation.Öğe Uric acid-lymphocyte ratios and myocardial damage parameters in ST elevated myocardial infarction(Rabia YILMAZ, 2020) Günay, Nahide Ekici; Baktır, Ahmet Oğuz; Çakır, İşıl; Muhtaroğlu, Sabahattin; Nisari, MustafaAbstractBackground/Aims:Studies recently have demonstrated the association between the major cardiovascular poor outcomes and uricacid (UA), the neutrophil/lymphocyteratio (NLR), lymphocyte counts. Barely, the relation between uricacid-to-lymphocyteratio (UALR) levels and inflammatory markers in ST elevated myocardial infarction (STEMI) hasn’t been investigated yet. In our current study, we tried to investigate whether alterations UALR levels between patients unstable angina pectoris (UAP) patients and STEMI patients who underwent primary percutaneous coronary intervention (PCI). In this context, were searched that altherelations of UALR with myocardial injury markers (troponin I, creatine kinase-MB), inflammatory markers (C-reactive protein, and covariaties of blood count), patients' lipid profiles and myocardial contractility.Methods:A total of 346 STEMI and UAP patients were enrolled in this retrospectively study. T-test or Mann Witney U test was used to see the significant differences. We found independent predictive factors for UA, NLR, and UALR for STEMI and ROC analyses was performed for these parameters. Results:We showed significant differences between UA, NLR and UALR levels (P<0.005)between STEMI and UAP patients. We determined the optimal cut-off points as: 6.05mg/dL for UA (UAC 0.561, specifity%50, sensitivity%72); 0.179 for UALR (UAC 0.913, specifity%96,sensitivity %92) ;2.3 for NLR (UAC 0.395, specifity %82, sensitivity %88). Conclusions: For the first time in the literature, it has been demonstrated that UALR is a distinct parameter associated with troponin I levels and myocardial contractility, and is more sensitive and specific than the NLR, UA and CRP parameters routinely used in STEMI.
