Kesim, BertanÜlger, Seda TezcanCudal, HamzaAslan, GönülErsoy, LeylaArslan, TuğrulKüçük, Mustafa Öner2025-02-242025-02-2420212146-176710.5577/intdentres.2021.vol11.no3.7https://doi.org/10.5577/intdentres.2021.vol11.no3.7https://search.trdizin.gov.tr/tr/yayin/detay/509261https://hdl.handle.net/20.500.14440/334Aim: The objective of this study was to evaluate the prevalence of\rEnterococcus faecalis in samples of oral rinse and tongue dorsum of\rendodontic patients with secondary/persistent infections (EPSI) using the\rPCR method.\rMethodology: Oral rinse samples (ORS) and tongue swab samples (TSS)\rof 22 patients (EPSI group) and 32 healthy individuals (control group) were\rcollected. DNA isolation from the TSS and ORS samples was performed\rusing the modified classical phenol-chloroform and chloroform method. To\rdetect E. faecalis strains directly from the TSS and ORS samples, the 310\rbase pair (bp) segment of the 16S rDNA of the E. faecalis genome was\ramplified by PCR using specific primers. The prevalence of E. faecalis was\rcompared between healthy and sick individuals using the Chi-square test,\rsignificance was set at p<0.05.\rResults: In the ORS samples, there was a significant difference between\rthe healthy individuals (n = 11, 34%) and the EPSI group (n = 15, 68%) in\rterms of the presence of E. faecalis (p = 0.026). In the TSS, the presence\rof E. faecalis was also investigated, and a significant difference was found\rbetween healthy individuals (n = 3, 9%) and the EPSI group (n = 11, 50%) (p\r= 0.001). In the EPSI group, no statistically significant difference was\rpresent in the prevalence rate of E. faecalis between the samples of ORS\r(68%) and TSS (50%) (p = 0.358).\rConclusion: The prevalence of E. faecalis was found to be statistically\rsignificantly higher in multi-site oral samples of a population with\rsecondary endodontic infection than healthy individuals.eninfo:eu-repo/semantics/openAccessDiş HekimliğiMikrobiyolojiArticle184318050926111