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    Release of mercury from amalgam filling and its relationship with metallothionein and superoxide dismutase
    (Istanbul Univ Press, Istanbul Univ Rectorate, 2023) Gul, Pinar; Karataş, Özcan; Sagsoz, Omer; Askin, Seda; Turkeri, Ozge; Kiziltunc, Ahmet
    Purpose This study aims at determining the amount of mercury released over time from amalgam after treatment in healthy subjects and to examine the relation of mercury with serum MT-1 and SOD-1 levels.Materials and Methods Amalgam filling was applied to the 15 subjects aged 19-22 years and blood samples were collected before treatment and 1 day, 7 days, 21 days and 35 days after treatment. Mercury analysis of serum samples was performed using Inductively Coupled Plasma Mass Spectrometry (ICP-MS). In addition, MT-1 and SOD-1 levels in serum samples were measured using commercial enzyme-linked immunosorbent assay (ELISA). Friedman test and Spearman's correlation analysis was performed to analyse the data. p value was interpreted in significance level of 0.05.Results As a result of the analysis for MT-1, it was found that the values decreased over time and this decrease was statistically significant after 21 days (p<0.05). In addition, it was found that SOD-1 decreased over time, but this decrease was not statistically significant . In terms of released mercury, there was no statistically significant difference among the values of mercury released over time . According to the results of correlation analysis, no statistically significant relationship was found among the variables.Conclusion The results of the present study indicated that the amount of mercury released from the tested amalgam were found to be tolerable and no significant relationship was found between MT-1 and SOD-1.
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    THE EFFECT OF ER,CR:YSGG LASER CONDITIONING ON DENTIN BOND STRENGTH AND NANOLEAKAGE OF UNIVERSAL ADHESIVE SYSTEMS: AN IN VITRO STUDY
    (Quintessence Publishing Co Inc, 2023) Karataş, Özcan; Sagsoz, Omer; Kapti, Zeynep Sumeyye
    This study investigated the effect of erbium, chromium: yttrium-scandium-gallium-garnet (Er,CrYSGG) laser conditioning on dentin bond strength and nanoleakage of different universal and self-etch adhesives. A total of 84 intact human third molar teeth were cut at the dentin level, and half of them were laser conditioned. The specimens were divided into three groups; two different universal resins and one self-etch adhesive resin were applied and composite resin restorations were created. For the microtensile bond strength (mu TBS) test, 20 microspecimens were prepared from the laser and control group of each adhesive (n = 20) and tested with a universal device. For nanoleakage observation, 10 specimens were prepared from each group (n = 10) and stored in silver nitrate solution, and then the amount of nanoleakage was analyzed by field emission scanning electron microscopy (FESEM). Data were analyzed with two-way ANOVA, Tukey's honestly significant difference, and chi-square tests. The mean dentin bond strength of all adhesives in the laser groups was found to be statistically significantly lower than those of the control groups (P < .05). No difference was found between the mean bond strength of the adhesives in the laser and control groups (P > .05). Higher nanoleakage was observed in all adhesives in the laser groups compared to the control groups (P < .05). Irradiation of the dentin surface with Er,Cr:YSGG could adversely affect the mu TBS and nanoleakage, likely by affecting the structure of the hybrid layer.