Yazar "Cudal, Hamza" seçeneğine göre listele

Listeleniyor 1 - 4 / 4
  • [ X ]
    Öğe
    Amplicon-based next-generation sequencing for comparative analysis of root canal microbiome of teeth with primary and persistent/secondary endodontic infections
    (Springer Heidelberg, 2023) Kesim, Bertan; Ulger, Seda Tezcan; Aslan, Gonul; Cudal, Hamza; Ustun, Yakup; Kucuk, Mustafa Oner
    ObjectivesTo compare the root canal microbiome profiles of primary and persistent/secondary infections using high-throughput sequencing with the help of a reliable bioinformatics algorithm.Materials and methodsRoot canal samples of 10 teeth in the primary endodontic infection (PEI) group and 10 teeth in the persistent/secondary endodontic infection (SEI) group were included resulting in a total of 20 samples. After DNA extraction from the samples, sequencing was performed on the Illumina MiSeq platform. Pair-end Illumina reads were imported to QIIME 2; amplicon sequence variants (ASVs) generated by DADA2 were mapped to GreenGenes database. Weighted UniFrac distances were calculated and principal coordinates analysis (PCoA) was used to compare beta diversity patterns. The multiple response permutation procedure (MRPP), the analysis of similarities (ANOSIM), and permutational multivariate analysis of variance (adonis) were conducted for testing group differences. Linear discriminant analysis effect size (LEfSe) analysis was utilized to identify differentially abundant taxa between the groups. The linear discriminant analysis (LDA) score threshold was set to 4.0.ResultsWithin the Gram-negative facultative anaerobic Gammaproteobacteria class outgroup, two orders (Pasteurellales, Vibrionales) and two families (Pasteurellaceae, Vibrionaceae) were significantly more abundant in the PEI group, whereas Gram-positive bacteria, Actinomycetales order, and Gram-positive anaerobic taxa, one genus (Olsenella) and one species (Olsenella uli), were identified as significantly more abundant in the SEI group.ConclusionsA few taxa were differentially abundant within either the PEI or SEI group.
  • [ X ]
    Öğe
    Effects of Intracanal Medicaments on the Measurement Accuracy of Four Apex Locators: An In Vitro Study
    (Galenos Publ House, 2023) Cudal, Hamza; Aslan, Tugrul; Kesim, Bertan
    Objective: This study aimed to examine the effect of various intracanal medicaments on the precision of four electronic apex locators (EALs). Materials and Methods: This study comprised 160 maxillary central incisors. After root canal preparation, a 25-K file was carefully introduced into the root canal until it was perceptible through the apex, and 0.5 mm was subtracted from this amount to determine the actual length. Samples were divided into four groups: calcium hydroxide, double antibiotic paste, triple antibiotic paste, and the control groups. Intracanal medications were removed with EDTA ten days later, and electronic measurements were performed. Chi-square (chi(2)) test was used to assess the precision of EALs within the margins of +/- 0.5 and +/- 1 mm (alpha=0.05). Results: The accuracies of the four EALs did not vary significantly for different intracanal drugs within the bounds of +/- 0.5 and +/- 1 mm (chi(2) tests, p>0.05). Conclusion: The EALs used in this study obtained reliable readings of working length in the context of varying intracanal medications.
  • [ X ]
    Öğe
    Effects of three different embedding media on the accuracy of different electronic apex locators: An in vitro study
    (Bayrakol Medical Publisher, 2022) Cudal, Hamza; Kesim, Bertan; Aslan, Tugrul
    Aim: In this study, we aimed to evaluate in vitro the measurement accuracy of three electronic apex locators (EALs) in three different embedding media. Material and Methods: Thirty maxillary central incisors were included (N = 30). The specimens were decoronated at cement-enamel junction, and the soft tissues in root canals were removed. The samples were randomly divided into three groups (n = 10). The specimens were embedded in alginate (group A), gelatin (group B) and agar agar (group C), leaving the coronal 2 mm of teeth exposed. Electronic lengths (ELs) of root canals of samples were measured using Raypex 6 (VDW, Munich, Germany), Apex ID (SybronEndo, Glendora, USA) and Ipex 2 (NSK Inc., Kanuma, Japan). In all groups, actual length (AL) values subtracted from EL values, and EL-AL values were recorded. Results: The EL-AL values for each embedding media were classified within an error range of +/- 0.5 mm and +/- 1 mm, and the chi-square (chi 2) test was used to compare the percentage of acceptable measurements of three EALs (alpha = 0.05). The measurements of Raypex 6 in agar agar medium were statistically different compared with those in alginate and gelatin media, within the error margin of +/- 0.5 mm (p < 0.05). Both in alginate and gelatin media, no statistically significant difference was observed in percentages of acceptable measurements with Raypex 6, Apex ID and Ipex 2 within two ranges of error (p > 0.05). Discussion: Within the limitations of this study, alginate and gelatin can be used safely as embedding media in studies investigating the in vitro measurement accuracy of EALs.
  • [ X ]
    Öğe
    Molecular detection of E. faecalis in oral samples of a population associated with secondary endodontic infection
    (2021) Kesim, Bertan; Ülger, Seda Tezcan; Cudal, Hamza; Aslan, Gönül; Ersoy, Leyla; Arslan, Tuğrul; Küçük, Mustafa Öner
    Aim: The objective of this study was to evaluate the prevalence of\rEnterococcus faecalis in samples of oral rinse and tongue dorsum of\rendodontic patients with secondary/persistent infections (EPSI) using the\rPCR method.\rMethodology: Oral rinse samples (ORS) and tongue swab samples (TSS)\rof 22 patients (EPSI group) and 32 healthy individuals (control group) were\rcollected. DNA isolation from the TSS and ORS samples was performed\rusing the modified classical phenol-chloroform and chloroform method. To\rdetect E. faecalis strains directly from the TSS and ORS samples, the 310\rbase pair (bp) segment of the 16S rDNA of the E. faecalis genome was\ramplified by PCR using specific primers. The prevalence of E. faecalis was\rcompared between healthy and sick individuals using the Chi-square test,\rsignificance was set at p<0.05.\rResults: In the ORS samples, there was a significant difference between\rthe healthy individuals (n = 11, 34%) and the EPSI group (n = 15, 68%) in\rterms of the presence of E. faecalis (p = 0.026). In the TSS, the presence\rof E. faecalis was also investigated, and a significant difference was found\rbetween healthy individuals (n = 3, 9%) and the EPSI group (n = 11, 50%) (p\r= 0.001). In the EPSI group, no statistically significant difference was\rpresent in the prevalence rate of E. faecalis between the samples of ORS\r(68%) and TSS (50%) (p = 0.358).\rConclusion: The prevalence of E. faecalis was found to be statistically\rsignificantly higher in multi-site oral samples of a population with\rsecondary endodontic infection than healthy individuals.