Amplicon‑based next‑generation sequencing for comparative analysis of root canal microbiome of teeth with primary and persistent/ secondary endodontic infections

Bertan KESİM | Mustafa Öner KÜÇÜK

Abstract Objectives To compare the root canal microbiome profiles of primary and persistent/secondary infections using highthroughput sequencing with the help of a reliable bioinformatics algorithm. Materials and methods Root canal samples of 10 teeth in the primary endodontic infection (PEI) group and 10 teeth in the persistent/secondary endodontic infection (SEI) group were included resulting in a total of 20 samples. After DNA extraction from the samples, sequencing was performed on the Illumina MiSeq platform. Pair-end Illumina reads were imported to QIIME 2; amplicon sequence variants (AS ...Daha fazlası